Real Review,are directed against the protein encoded by the gene GAP43

GAP43 antibody research is a critical area within neuroscience, focusing on a protein integral to neuronal development, regeneration, and function. Growth Associated Protein 43 (GAP-43), also known as neuromodulin, is a major component of the motile growth cones that form the tips of elongating axons. Its expression is significantly upregulated during periods of active axonal growth, making it a key marker for developing or regenerating neurons. Understanding the precise roles of GAP-43 and utilizing specific antibodies to detect it are paramount for advancing our knowledge of neural pathways and developing therapeutic strategies for neurological disorders.

The search_keyword "gap43 antibody" has revealed a diverse range of commercially available antibodies, each with specific characteristics and validated applications. These antibodies are essential tools for researchers, enabling them to detect GAP43 protein expression in various biological contexts. The utility of these antibodies extends across a broad spectrum of scientific applications, including Immunohistochemistry (IHC), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Flow Cytometry. This versatility allows for the investigation of GAP-43 at different levels, from tissue sections to cellular and subcellular compartments.

Entities and Their Significance:

At the core of this research is the GAP43 protein itself. This protein is intrinsically linked to nerve growth and plays a crucial role in axonal growth and guidance. Its involvement is particularly pronounced during neural development and after injury, where it facilitates neuronal pathfinding and branching. The gene encoding GAP-43 in humans may also be known by other designations, highlighting the importance of precise terminology in scientific literature.

LSI Keywords and Their Applications:

Several LSI keywords emerge from the analysis of the provided data, shedding light on the functional and structural aspects of GAP-43 and its detection. The protein is described as an intracellular growth-associated protein that assists in neuronal development. It is a common marker of differentiating neurons, and its expression is elevated during axon growth. Furthermore, GAP43 antibody research often involves studying its presence in the axonal membrane of neuronal cells, differentiating it from other neuronal markers like MAP2, which stains dendrites and perikarya.

Variations and Specific Antibody Information:

The landscape of GAP-43 antibodies is rich with variations, catering to different research needs. Researchers can find antibodies that are rabbit monoclonal antibody, mouse monoclonal antibody, or sheep polyclonal antibody. Specific clones, such as [EP890Y], [GAP-7B10], and [3H14], are frequently cited, indicating specific epitopes targeted by these antibodies. The host species for antibody production also varies, including rabbit, mouse, and sheep, with reactivity often extending to multiple species such as human, mouse, rat, and even bovine, canine, chicken, feline, hamster, porcine, primate, reptilian, and other species. This broad species reactivity allows for comparative studies across different model organisms.

Verifiable Information and Parameters:

Many of the GAP43 antibody products are associated with specific catalog numbers (e.g., ab75810, ab129990, GTX127937, 16971-1-AP, A19055, A85393, ANR-016, E-AB-61918, NB300-143, MBS179756), enabling researchers to easily identify and procure the desired reagents. The typical molecular weight associated with GAP-43 is around 43 kDa, which is a key parameter for validation in Western Blot experiments. Dilution ratios for IHC, such as 1:5000 for the GAP43 Rabbit mAb (A19055) in mouse brain tissue, provide crucial starting points for experimental optimization. Some antibodies are also described as being unconjugated, meaning they do not have a label attached and require a secondary detection system.

Search Intent Integration:

The comprehensive nature of GAP43 antibody research means that these antibodies can be used in several scientific applications. For instance, the antibody [EP890Y] is specifically noted as a rabbit monoclonal antibody detecting GAP43 in various applications. The fundamental understanding that GAP43 is associated with nerve growth drives much of this investigation. Furthermore, antibodies are directed against the protein encoded by the gene GAP43, emphasizing the specificity of these reagents. The fact that GAP43 is an intracellular growth-associated protein informs experimental design, particularly regarding cell lysis and protein extraction. The ability of a GAP43 antibody to detect GAP43 protein expression is the primary goal of its use. Researchers often seek antibodies that can be used in a variety of model species, expanding the scope of their studies. The specific antibody LS-C180068